Proteomic comparison of the EWS-FLI1 expressing cells EF with NIH-3T3 and actin remodeling effect of (R/W)₉ cell-penetrating peptide.

ABSTRACT: EWS-FLI1 expression in NIH-3T3 fibroblasts has a profound impact on the phenotype, resulting in the cytoskeleton and adhesive capacity disorganization (EF cells). Besides this, (R/W)₉, a cell-penetrating peptide (CPP), has an intrinsic actin remodeling activity in EF cells. To evaluate the impact of the oncogenic protein EWS-FLI1 on proteins expression levels, a quantitative comparison of tumoral EF and non-tumoral 3T3 proteomes was performed. Then to see if we could link the EWS-FLI1 oncogenic transformation to the phenotype reversion induced by (R/W)₉, (R/W)₉ influence on EF cells proteome was assessed. To our knowledge no such ⿿CPPomic⿿ study has been performed before.

Biological significance

Up to now very few global quantitative proteomic studie

SUBMITTER: Clavier S 

PROVIDER: S-EPMC5988571 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature