Comparing Sanger sequencing and high-throughput metabarcoding for inferring photobiont diversity in lichens.

ABSTRACT: The implementation of HTS (high-throughput sequencing) approaches is rapidly changing our understanding of the lichen symbiosis, by uncovering high bacterial and fungal diversity, which is often host-specific. Recently, HTS methods revealed the presence of multiple photobionts inside a single thallus in several lichen species. This differs from Sanger technology, which typically yields a single, unambiguous algal sequence per individual. Here we compared HTS and Sanger methods for estimating the diversity of green algal symbionts within lichen thalli using 240 lichen individuals belonging to two species of lichen-forming fungi. According to HTS data, Sanger technology consistently yielded the most abundant photobiont sequence in the sample. However, if the second most abundant photobiont e

SUBMITTER: Paul F 

PROVIDER: S-EPMC5988838 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature