Absence of hybridization with the wild-type and mutant rpoB probes in the Genotype MTBDRplus assay detects 'disputed' rifampicin mutations.

ABSTRACT:

Objective

Mycobacterium tuberculosis isolates that fail to hybridize to at least one rpoB wild-type or any mutation probe on the Genotype MTBDRplus strip are assumed to be rifampicin-resistant. However, the precise mutation(s) are unknown. We sought to identify the mutations in isolates with such hybridization patterns and determine if the mutations are associated with resistance to rifampicin.

Methods

In this study, 275 M. tuberculosis isolates were screened with the Genotype MTBDRplus assay to identify isolates with the hybridization pattern. These isolates were sequenced and their minimum inhibitory concentrations (MIC) determined using the Bactec MGIT 960 system.

Results

Among the 275 isolates tested, 15 (6%) isolates with the hybridization pattern were identified

SUBMITTER: Abanda NN 

PROVIDER: S-EPMC5988914 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature