Biomonitoring for traditional herbal medicinal products using DNA metabarcoding and single molecule, real-time sequencing.

ABSTRACT: Global concerns have been paid to the potential hazard of traditional herbal medicinal products (THMPs). Substandard and counterfeit THMPs, including traditional Chinese patent medicine, health foods, dietary supplements, etc. are potential threats to public health. Recent marketplace studies using DNA barcoding have determined that the current quality control methods are not sufficient for ensuring the presence of authentic herbal ingredients and detection of contaminants/adulterants. An efficient biomonitoring method for THMPs is of great needed. Herein, metabarcoding and single-molecule, real-time (SMRT) sequencing were used to detect the multiple ingredients in Jiuwei Qianghuo Wan (JWQHW), a classical herbal prescription widely used in China for the last 800 years. Reference experiment

SUBMITTER: Xin T 

PROVIDER: S-EPMC5990340 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature