Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis.

ABSTRACT: Epidemiological studies have demonstrated an increased leukemia incidence following ionizing radiation exposure, but to date, the target cells and underlying mechanisms of radiation leukemogenesis remain largely unidentified. We engineered a mouse model carrying a different fluorescent marker on each chromosome 2, located inside the minimum deleted region occurring after radiation exposure and recognized as the first leukemogenic event. Using this tailored model, we report that following radiation exposure, more than half of asymptomatic CBA Sfpi1 GFP/mCh mice presented with expanding clones of preleukemic hematopoietic cells harboring a hemizygous interstitial deletion of chromosome 2. Moreover, following isolation of preleukemic hematopoietic stem and progenitor cells irradiated in their

SUBMITTER: Verbiest T 

PROVIDER: S-EPMC5990525 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature