Metabolic Patterns in Spirodela polyrhiza Revealed by ¹⁵N Stable Isotope Labeling of Amino Acids in Photoautotrophic, Heterotrophic, and Mixotrophic Growth Conditions.

ABSTRACT: In this study we describe a [¹⁵N] stable isotopic labeling study of amino acids in Spirodela polyrhiza (common duckweed) grown under three different light and carbon input conditions which represent unique potential metabolic modes. Plants were grown with a light cycle, either with supplemental sucrose (mixotrophic) or without supplemental sucrose (photoautotrophic) and in the dark with supplemental sucrose (heterotrophic). Labeling patterns, pool sizes (both metabolically active and inactive), and kinetics/turnover rates were estimated for 17 of the proteinogenic amino acids. Estimation of these parameters followed several overall trends. First, most amino acids showed plateaus in labeling patterns of <100% [¹⁵N]-labeling, indicating the possibility of a large

SUBMITTER: Evans EM 

PROVIDER: S-EPMC5990592 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature