The whole transcriptome effects of the PPARα agonist fenofibrate on livers of hepatocyte humanized mice.

ABSTRACT:

Background

The role of PPARα in gene regulation in mouse liver is well characterized. However, less is known about the role of PPARα in human liver. The aim of the present study was to better characterize the impact of PPARα activation on gene regulation in human liver. To that end, chimeric mice containing hepatocyte humanized livers were given an oral dose of 300 mg/kg fenofibrate daily for 4 days. Livers were collected and analyzed by hematoxilin and eosin staining, qPCR, and transcriptomics. Transcriptomics data were compared with existing datasets on PPARα activation in normal mouse liver, human primary hepatocytes, and human precision cut liver slices.

Results

Of the different human liver models, the gene expression profile of hepatocyte humanized livers most closely r

SUBMITTER: de la Rosa Rodriguez MA 

PROVIDER: S-EPMC5991453 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature