Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis.

ABSTRACT:

Background

Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called 'cellulosome'. Hence, we proposed a "biomimic operon" concept for making an artificial cellulosome which can be used as a promising tool for the expression of cellulosomal enzymes in Bacillus subtilis.

Results

According to the proteomic analysis of Clostridium thermocellum ATCC27405 induced by Avicel or cellobiose, we selected eight highly expressed cellulosomal genes including a scaffoldin protein gene (cipA), a cell-surface anchor gene (sdbA), two exoglucanase genes (celK and celS), two endoglucanase genes (

SUBMITTER: Chang JJ 

PROVIDER: S-EPMC5991470 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature