Comprehensive viral enrichment enables sensitive respiratory virus genomic identification and analysis by next generation sequencing.

ABSTRACT: Next generation sequencing (NGS) technologies have revolutionized the genomics field and are becoming more commonplace for identification of human infectious diseases. However, due to the low abundance of viral nucleic acids (NAs) in relation to host, viral identification using direct NGS technologies often lacks sufficient sensitivity. Here, we describe an approach based on two complementary enrichment strategies that significantly improves the sensitivity of NGS-based virus identification. To start, we developed two sets of DNA probes to enrich virus NAs associated with respiratory diseases. The first set of probes spans the genomes, allowing for identification of known viruses and full genome sequencing, while the second set targets regions conserved among viral families or genera, prov

SUBMITTER: O'Flaherty BM 

PROVIDER: S-EPMC5991510 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature