Arrayed CRISPR screen with image-based assay reliably uncovers host genes required for coxsackievirus infection.

ABSTRACT: Pooled CRISPR screens based on lentiviral systems have been widely applied to identify the effect of gene knockout on cellular phenotype. Although many screens were successful, they also have the limitation that genes conferring mild phenotypes or those essential for growth can be overlooked, as every genetic perturbation is incorporated in the same population. Arrayed screens, on the other hand, incorporate a single genetic perturbation in each well and could overcome these limitations. However, arrayed screens based on siRNA-mediated knockdown were recently criticized for low reproducibility caused by incomplete inhibition of gene expression. To overcome these limitations, we developed a novel arrayed CRISPR screen based on a plasmid library expressing a single guide RNA (sgRNA) and disr

SUBMITTER: Kim HS 

PROVIDER: S-EPMC5991512 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature