A comparative transcriptional landscape of maize and sorghum obtained by single-molecule sequencing.

ABSTRACT: Maize and sorghum are both important crops with similar overall plant architectures, but they have key differences, especially in regard to their inflorescences. To better understand these two organisms at the molecular level, we compared expression profiles of both protein-coding and noncoding transcripts in 11 matched tissues using single-molecule, long-read, deep RNA sequencing. This comparative analysis revealed large numbers of novel isoforms in both species. Evolutionarily young genes were likely to be generated in reproductive tissues and usually had fewer isoforms than old genes. We also observed similarities and differences in alternative splicing patterns and activities, both among tissues and between species. The maize subgenomes exhibited no bias in isoform generation; however,

SUBMITTER: Wang B 

PROVIDER: S-EPMC5991521 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature