Target sequence capture of nuclear-encoded genes for phylogenetic analysis in ferns.

ABSTRACT:

Premise of the study

Until recently, most phylogenetic studies of ferns were based on chloroplast genes. Evolutionary inferences based on these data can be incomplete because the characters are from a single linkage group and are uniparentally inherited. These limitations are particularly acute in studies of hybridization, which is prevalent in ferns; fern hybrids are common and ferns are able to hybridize across highly diverged lineages, up to 60 million years since divergence in one documented case. However, it not yet clear what effect such hybridization has on fern evolution, in part due to a paucity of available biparentally inherited (nuclear-encoded) markers.

Methods

We designed oligonucleotide baits to capture 25 targeted, low-copy nuclear markers from a sample of 24

SUBMITTER: Wolf PG 

PROVIDER: S-EPMC5991577 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature