Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing.

ABSTRACT: Regular blood transfusion is the cornerstone of care for patients with red blood cell (RBC) disorders such as thalassaemia or sickle-cell disease. With repeated transfusion, alloimmunisation often occurs due to incompatibility at the level of minor blood group antigens. We use CRISPR-mediated genome editing of an immortalised human erythroblast cell line (BEL-A) to generate multiple enucleation competent cell lines deficient in individual blood groups. Edits are combined to generate a single cell line deficient in multiple antigens responsible for the most common transfusion incompatibilities: ABO (Bombay phenotype), Rh (Rh_null), Kell (K₀), Duffy (Fy_null), GPB (S-s-U-). These cells can be differentiated to generate deformable reticulocytes, illustrat

SUBMITTER: Hawksworth J 

PROVIDER: S-EPMC5991592 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature