Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens.

ABSTRACT: Vibrio natriegens constitutes one of the fastest-growing nonpathogenic bacteria and a potential novel workhorse for many biotechnological applications. Here, we report the development of a Vibrio-based cell-free protein synthesis system (CFPS). Specifically, up to 0.4 g L^-1 eGFP could be successfully synthesized in small-scale batch reactions using cell-free extract obtained from fast-growing V. natriegens cultures. Versatile CFPS system characterization attained by combining the analyses of key metabolites for translation and ribosomes revealed limitations regarding rRNA stability and critical substrate consumption (e.g., amino acids). Alternatively, rRNA showed increased stability by inducing Mg²⁺homeostasis in the reaction. Although the enormou

SUBMITTER: Failmezger J 

PROVIDER: S-EPMC5992293 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature