Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ.

ABSTRACT: Electron microscopy imaging of macromolecular complexes in their native cellular context is limited by the inherent difficulty to acquire high-resolution tomographic data from thick cells and to specifically identify elusive structures within crowded cellular environments. Here, we combined cryo-fluorescence microscopy with electron cryo-tomography of vitreous sections into a coherent correlative microscopy workflow, ideal for detection and structural analysis of elusive protein assemblies in situ. We used this workflow to address an open question on BAR-domain coating of yeast plasma membrane compartments known as eisosomes. BAR domains can sense or induce membrane curvature, and form scaffold-like membrane coats in vitro. Our results demonstrate that in cells, the BAR protein Pil1 locali

SUBMITTER: Bharat TAM 

PROVIDER: S-EPMC5992340 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature