Enhancing Electrotransfection Efficiency through Improvement in Nuclear Entry of Plasmid DNA.

ABSTRACT: The nuclear envelope is a physiological barrier to electrogene transfer. To understand different mechanisms of the nuclear entry for electrotransfected plasmid DNA (pDNA), the current study investigated how manipulation of the mechanisms could affect electrotransfection efficiency (eTE), transgene expression level (EL), and cell viability. In the investigation, cells were first synchronized at G2-M phase prior to electrotransfection so that the nuclear envelope breakdown (NEBD) occurred before pDNA entered the cells. The NEBD significantly increased the eTE and the EL while the cell viability was not compromised. In the second experiment, the cells were treated with a nuclear pore dilating agent (i.e., trans-1,2-cyclohexanediol). The treatment could increase the EL, but had only minor effe

SUBMITTER: Cervia LD 

PROVIDER: S-EPMC5992438 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature