Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

ABSTRACT: In the intraflagellar transport (IFT) machinery, the IFT-B and IFT-A complexes mediate anterograde and retrograde ciliary protein trafficking, respectively. Among the 16 subunits of the IFT-B complex, several subunits are essential for ciliogenesis, whereas others, which are associated peripherally with the complex, are dispensable for ciliogenesis but play a role in protein trafficking. IFT22-knockout (KO) cells established in this study demonstrated no defects in ciliogenesis or ciliary protein trafficking. In stark contrast, IFT70A and IFT70B double-knockout cells did not form cilia, even though IFT70 is associated peripherally with the IFT-B complex via the IFT52-IFT88 dimer, and other IFT-B subunits assembled at the ciliary base in the absence of IFT70. Exogenous

SUBMITTER: Takei R 

PROVIDER: S-EPMC5992529 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature