Metabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation.

ABSTRACT:

Background

Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the accumulation of endogenous S-adenosylmethionine (SAM). To overcome the methionine dependent stimulation of CPC production, the methionine cycle of A. chrysogenum was reconstructed by metabolic engineering.

Results

Three engineered strains were obtained by overexpressing the SAM synthetase gene AcsamS and the cystathionine-γ-lyase gene mecB, and disrupting a SAM dependent methyltransferase gene Acppm1, respectively. Overexpression of AcsamS resulted in fourfold

SUBMITTER: Liu J 

PROVIDER: S-EPMC5992653 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature