Manipulation of two regulatory genes for efficient production of chromomycins in Streptomyces reseiscleroticus.

ABSTRACT:

Background

Regulatory genes play critical roles in natural product biosynthetic pathways. Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain for production of these molecules by manipulating the regulatory genes.

Results

A putative but silent chromomycin biosynthetic gene cluster was discovered in Streptomyces reseiscleroticus. Heterologous expression of the ketosynthase, chain length factor, and acyl carrier protein in Streptomyces lividans confirmed that they are responsible for the assembly of a decaketide. Two regulatory genes are present in this gene cluster, including SARP-type activator SrcmRI and PadR-like repressor SrcmRII. Either overexpression of SrcmRI or disruption of SrcmRII

SUBMITTER: Sun L 

PROVIDER: S-EPMC5992853 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature