Cdc42 Regulates Neuronal Polarity during Cerebellar Axon Formation and Glial-Guided Migration.

ABSTRACT: CNS cortical histogenesis depends on polarity signaling pathways that regulate cell adhesion and motility. Here we report that conditional deletion of the Rho GTPase Cdc42 in cerebellar granule cell precursors (GCPs) results in abnormalities in cerebellar foliation revealed by iDISCO clearing methodology, a loss of columnar organization of proliferating GCPs in the external germinal layer (EGL), disordered parallel fiber organization in the molecular layer (ML), and a failure to extend a leading process and form a neuron-glial junction during migration along Bergmann glia (BG). Notably, GCPs lacking Cdc42 had a multi-polar morphology and slowed migration rate. In addition, secondary defects occurred in BG development and organization, especially in the lateral cerebellar hemi

SUBMITTER: Govek EE 

PROVIDER: S-EPMC5993048 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature