High molecular weight hyaluronic acid: a two-pronged protectant against infection of the urogenital tract?

ABSTRACT:

Objectives

Recurrent urinary tract infections are associated with uropathogenic Escherichia coli (UPEC) ascending and infecting the urinary tract. Antibiotics provide only symptomatic relief, not prevent recurrence. Clinical evidence suggests that intravesical glycosaminoglycan therapy, such as hyaluronic acid (HA), helps reduce UTI recurrence. This has been investigated here using in vitro systems modelling the urogenital tract tissues.

Methods

RT4 bladder cells were preconditioned with high molecular weight HA (> 1500 kDa) at 2 mg mL^-1 and challenged with UPEC to analyse barrier protection and bacterial adherence. Untreated and HA-preconditioned VK2 E6/E7 vaginal cells were challenged with E. coli flagellin (50 ng mL^-1) to mimic b

SUBMITTER: Mowbray CA 

PROVIDER: S-EPMC5993165 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature