Iron deficiency impairs contractility of human cardiomyocytes through decreased mitochondrial function.

ABSTRACT:

Aims

Iron deficiency is common in patients with heart failure and associated with a poor cardiac function and higher mortality. How iron deficiency impairs cardiac function on a cellular level in the human setting is unknown. This study aims to determine the direct effects of iron deficiency and iron repletion on human cardiomyocytes.

Methods and results

Human embryonic stem cell-derived cardiomyocytes were depleted of iron by incubation with the iron chelator deferoxamine (DFO). Mitochondrial respiration was determined by Seahorse Mito Stress test, and contractility was directly quantified using video analyses according to the BASiC method. The activity of the mitochondrial respiratory chain complexes was examined using spectrophotometric enzyme assays. Four days of iron de

SUBMITTER: Hoes MF 

PROVIDER: S-EPMC5993224 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature