Atlas of the Immune Cell Repertoire in Mouse Atherosclerosis Defined by Single-Cell RNA-Sequencing and Mass Cytometry.

ABSTRACT:

Rationale

Atherosclerosis is a chronic inflammatory disease that is driven by the interplay of pro- and anti-inflammatory leukocytes in the aorta. Yet, the phenotypic and transcriptional diversity of aortic leukocytes is poorly understood.

Objective

We characterized leukocytes from healthy and atherosclerotic mouse aortas in-depth by single-cell RNA-sequencing and mass cytometry (cytometry by time of flight) to define an atlas of the immune cell landscape in atherosclerosis.

Methods and results

Using single-cell RNA-sequencing of aortic leukocytes from chow diet- and Western diet-fed Apoe^-/- and Ldlr^-/- mice, we detected 11 principal leukocyte clusters with distinct phenotypic and spatial characteristics while the cellular repertoir

SUBMITTER: Winkels H 

PROVIDER: S-EPMC5993603 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature