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CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.

ABSTRACT: CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

SUBMITTER: Swings T

PROVIDER: S-EPMC5993718 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.

Swings Toon T Marciano David C DC Atri Benu B Bosserman Rachel E RE Wang Chen C Leysen Marlies M Bonte Camille C Schalck Thomas T Furey Ian I Van den Bergh Bram B Verstraeten Natalie N Christie Peter J PJ Herman Christophe C Lichtarge Olivier O Michiels Jan J

Nature communications 20180608 1

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering. ...[more]

PMID: 29884781

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