Impact of physical confinement on nuclei geometry and cell division dynamics in 3D spheroids.

ABSTRACT: Multicellular tumour spheroids are used as a culture model to reproduce the 3D architecture, proliferation gradient and cell interactions of a tumour micro-domain. However, their 3D characterization at the cell scale remains challenging due to size and cell density issues. In this study, we developed a methodology based on 3D light sheet fluorescence microscopy (LSFM) image analysis and convex hull calculation that allows characterizing the 3D shape and orientation of cell nuclei relative to the spheroid surface. By using this technique and optically cleared spheroids, we found that in freely growing spheroids, nuclei display an elongated shape and are preferentially oriented parallel to the spheroid surface. This geometry is lost when spheroids are grown in conditions of physical confinem

SUBMITTER: Desmaison A 

PROVIDER: S-EPMC5993719 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature