Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol.

ABSTRACT:

Background

The genus Bacillus includes a great variety of species with potential applications in biotechnology. While species such as B. subtilis or B. licheniformis are well-known and used to provide various products at industrial scale, other Bacillus species are less characterized and are not yet used in commercial processes. One reason for this is the fact that genetic manipulation of new isolates is usually complicated with conventional techniques which have to be adapted to each new strain. Even in well-established strains, the available transformation protocols often suffer from low efficiencies.

Results

In this paper, we provide a new broad host range E. coli/Bacillus shuttle vector, named pBACOV (Bacillus conjugation vector), that can be efficiently transferred to v

SUBMITTER: Heinze S 

PROVIDER: S-EPMC5994095 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature