Single-cell profiling of peanut-responsive T cells in patients with peanut allergy reveals heterogeneous effector T_H2 subsets.

ABSTRACT:

Background

The contribution of phenotypic variation of peanut-specific T cells to clinical allergy or tolerance to peanut is not well understood.

Objectives

Our objective was to comprehensively phenotype peanut-specific T cells in the peripheral blood of subjects with and without peanut allergy (PA).

Methods

We obtained samples from patients with PA, including a cohort undergoing baseline peanut challenges for an immunotherapy trial (Consortium of Food Allergy Research [CoFAR] 6). Subjects were confirmed as having PA, or if they passed a 1-g peanut challenge, they were termed high-threshold subjects. Healthy control (HC) subjects were also recruited. Peanut-responsive T cells were identified based on CD154 expression after 6 to 18 hours of stimulation with peanut extr

SUBMITTER: Chiang D 

PROVIDER: S-EPMC5994177 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature