Evaluation of the proliferation marker Ki-67 in gliomas: Interobserver variability and digital quantification.

ABSTRACT:

Background

The Ki-67 Labelling Index (LI) is used as an ancillary tool in glioma diagnostics. Interobserver variability has been reported and no precise guidelines are available. Nor is it known whether novel digital approaches would be an advantage. Our aim was to evaluate the inter- and intraobserver variability of the Ki-67 LI between two pathologists and between pathologists and digital quantification both in whole tumour slides and in hot spots using narrow but diagnostically relevant intervals.

Methods

In samples of 235 low and high grade gliomas, two pathologists (A and B) estimated the Ki-67 LI (5-10% intervals) for whole tumour slides and for hot spots. In 20 of the cases intraobserver variability was evaluated. For digital quantification (C) slides were scanned wit

SUBMITTER: Nielsen LAG 

PROVIDER: S-EPMC5994254 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature