Identification of a Recurrent LMO7-BRAF Fusion in Papillary Thyroid Carcinoma.

ABSTRACT: BACKGROUND:The BRAFV600E mutation is the most common driver in papillary thyroid carcinoma (PTC) tumors. In recent years, gene fusions have also been recognized as important drivers of cancer in PTC. Previous studies have suggested that thyroid tumors with fusion genes frequently display an aggressive course. These observations prompted further exploration of gene fusions in PTC tumors. The aim was to search for previously unrecognized gene fusions using thyroid tissue samples from PTC patients. METHODS:Gene fusions were analyzed in RNA sequencing data obtained from 12 PTC tumors and paired unaffected thyroid tissue samples. Candidate fusions were further filtered and validated using reverse transcriptase polymerase chain reaction, Sanger sequencing, and fluorescence in situ hybridization.

SUBMITTER: He H 

PROVIDER: S-EPMC5994666 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature