High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans.

ABSTRACT:

Purpose

Adherence capacity is one of the principal virulence factors of Streptococcus mutans, and adhesion virulence factors are controlled by small RNAs (sRNAs) at the post-transcriptional level in various bacteria. Here, we aimed to identify and decipher putative adhesion-related sRNAs in clinical strains of S. mutans.

Methodology

RNA deep-sequencing was performed to identify potential sRNAs under different adhesion conditions. The expression of sRNAs was analysed by quantitative real-time PCR (qRT-PCR), and bioinformatic methods were used to predict the functional characteristics of sRNAs.

Results

A total of 736 differentially expressed candidate sRNAs were predicted, and these included 352 sRNAs located on the antisense to mRNA (AM) and 384 sRNAs in intergenic reg

SUBMITTER: Zhu W 

PROVIDER: S-EPMC5994696 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature