Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi.

ABSTRACT: Although the genome of Trypanosoma cruzi, the causative agent of Chagas disease, was first made available in 2005, with additional strains reported later, the intrinsic genome complexity of this parasite (the abundance of repetitive sequences and genes organized in tandem) has traditionally hindered high-quality genome assembly and annotation. This also limits diverse types of analyses that require high degrees of precision. Long reads generated by third-generation sequencing technologies are particularly suitable to address the challenges associated with T. cruzi's genome since they permit direct determination of the full sequence of large clusters of repetitive sequences without collapsing them. This, in turn, not only allows accurate estimation of gene copy numbers but also circumvents

SUBMITTER: Berna L 

PROVIDER: S-EPMC5994713 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature