A 3D Human Renal Cell Carcinoma-on-a-Chip for the Study of Tumor Angiogenesis.

ABSTRACT: Tractable human tissue-engineered 3D models of cancer that enable fine control of tumor growth, metabolism, and reciprocal interactions between different cell types in the tumor microenvironment promise to accelerate cancer research and pharmacologic testing. Progress to date mostly reflects the use of immortalized cancer cell lines, and progression to primary patient-derived tumor cells is needed to realize the full potential of these platforms. For the first time, we report endothelial sprouting induced by primary patient tumor cells in a 3D microfluidic system. Specifically, we have combined primary human clear cell renal cell carcinoma (ccRCC) cells from six independent donors with human endothelial cells in a vascularized, flow-directed, 3D culture system ("ccRCC-on-a-chip"). The upre

SUBMITTER: Miller CP 

PROVIDER: S-EPMC5994779 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature