Metabolic engineering of Saccharomyces cerevisiae for overproduction of triacylglycerols.

ABSTRACT: Triacylglycerols (TAGs) are valuable versatile compounds that can be used as metabolites for nutrition and health, as well as feedstocks for biofuel production. Although Saccharomyces cerevisiae is the favored microbial cell factory for industrial production of biochemicals, it does not produce large amounts of lipids and TAGs comprise only ~1% of its cell dry weight. Here, we engineered S. cerevisiae to reorient its metabolism for overproduction of TAGs, by regulating lipid droplet associated-proteins involved in TAG synthesis and hydrolysis. We implemented a push-and-pull strategy by overexpressing genes encoding a deregulated acetyl-CoA carboxylase, ACC1^S659A/S1157A(ACC1**), as well as the last two steps of TAG formation: phosphatidic phosph

SUBMITTER: Ferreira R 

PROVIDER: S-EPMC5994799 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature