A novel chimaeric flocculation protein enhances flocculation in Saccharomyces cerevisiae.

ABSTRACT: Yeast flocculation is the reversible formation of multicellular complexes mediated by lectin-like cell wall proteins binding to neighbouring cells. Strong flocculation can improve the inhibitor tolerance and fermentation performance of yeast cells in second generation bioethanol production. The strength of flocculation increases with the size of the flocculation protein and is strain dependent. However, the large number of internal repeats in the sequence of FLO1 from Saccharomyces cerevisiae S288c makes it difficult to recombinantly express the gene to its full length. In the search for novel flocculation genes resulting in strong flocculation, we discovered a DNA sequence, FLONF, that gives NewFlo phenotype flocculation in S. cerevisiae CEN.PK 113-7D. The nucl

SUBMITTER: Westman JO 

PROVIDER: S-EPMC5994806 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature