The contributions of the actin machinery to endocytic membrane bending and vesicle formation.

ABSTRACT: Branched and cross-linked actin networks mediate cellular processes that move and shape membranes. To understand how actin contributes during the different stages of endocytic membrane reshaping, we analyzed deletion mutants of yeast actin network components using a hybrid imaging approach that combines live imaging with correlative microscopy. We could thus temporally dissect the effects of different actin network perturbations, revealing distinct stages of actin-based membrane reshaping. Our data show that initiation of membrane bending requires the actin network to be physically linked to the plasma membrane and to be optimally cross-linked. Once initiated, the membrane invagination process is driven by nucleation and polymerization of new actin filaments, independent of the degree of c

SUBMITTER: Picco A 

PROVIDER: S-EPMC5994895 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature