Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine.

ABSTRACT:

Background

To establish correlates of human immunity to the live plague vaccine (LPV), we analyzed parameters of cellular and antibody response to the plasminogen activator Pla of Y. pestis. This outer membrane protease is an essential virulence factor that is steadily expressed by Y. pestis.

Methodology/principal findings

PBMCs and sera were obtained from a cohort of naïve (n = 17) and LPV-vaccinated (n = 34) donors. Anti-Pla antibodies of different classes and IgG subclasses were determined by ELISA and immunoblotting. The analysis of antibody response was complicated with a strong reactivity of Pla with normal human sera. The linear Pla B-cell epitopes were mapped using a library of 15-mer overlapping peptides. Twelve peptides that reacted specifically with sera of vaccin

SUBMITTER: Feodorova VA 

PROVIDER: S-EPMC5995359 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature