X Chromosome Dosage Influences DNA Methylation Dynamics during Reprogramming to Mouse iPSCs.

ABSTRACT: A dramatic difference in global DNA methylation between male and female cells characterizes mouse embryonic stem cells (ESCs), unlike somatic cells. We analyzed DNA methylation changes during reprogramming of male and female somatic cells and in resulting induced pluripotent stem cells (iPSCs). At an intermediate reprogramming stage, somatic and pluripotency enhancers are targeted for partial methylation and demethylation. Demethylation within pluripotency enhancers often occurs at ESC binding sites of pluripotency transcription factors. Late in reprogramming, global hypomethylation is induced in a female-specific manner. Genome-wide hypomethylation in female cells affects many genomic landmarks, including enhancers and imprint control regions, and accompanies the reactivation of the inact

SUBMITTER: Pasque V 

PROVIDER: S-EPMC5995367 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature