MicroRNA-21 in cancer-associated fibroblasts supports lung adenocarcinoma progression.

ABSTRACT: Cancer-associated fibroblasts (CAFs) interact closely with cancer cells, supporting their growth and invasion. To investigate the role of microRNA-21 (miR-21) in lung adenocarcinoma, and especially in its CAF component, in situ hybridisation was applied to samples from 89 invasive lung adenocarcinoma cases. MiR-21 expression was observed in both cancer cells and CAFs. When the patients were stratified by expression, miR-21 levels in CAFs (n = 9), but not in cancer cells (n = 21), were inversely correlated with patient survival; patients with miR-21^high CAFs exhibited lower survival than those with miR-21^low CAFs. The underlying mechanism was investigated in vitro. Conditioned medium (CM) from A549 lung cancer cells increased miR-21 expression in MRC-5 and IMR-90 lung

SUBMITTER: Kunita A 

PROVIDER: S-EPMC5995955 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature