Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides.

ABSTRACT: A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification by NST, C5-Epi/2-OST and 6-OST₁ /6-OST₃ , thus resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST, and 6-OST.

SUBMITTER: Lu W 

PROVIDER: S-EPMC5996245 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature