Complement C5a Alters the Membrane Potential of Neutrophils during Hemorrhagic Shock.

ABSTRACT:

Background

Polymorphonuclear granulocytes (PMN) play a crucial role in host defense. Physiologically, exposure of PMN to the complement activation product C5a results in a protective response against pathogens, whereas in the case of systemic inflammation, excessive C5a substantially impairs neutrophil functions. To further elucidate the inability of PMN to properly respond to C5a, this study investigates the role of the cellular membrane potential of PMN in response to C5a.

Methods

Electrophysiological changes in cellular and mitochondrial membrane potential and intracellular pH of PMN from human healthy volunteers were determined by flow cytometry after exposure to C5a. Furthermore, PMN from male Bretoncelles-Meishan-Willebrand cross-bred pigs before and three hours after

SUBMITTER: Messerer DAC 

PROVIDER: S-EPMC5996468 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature