The Timing of Transcriptional Regulation in Synthetic Gene Circuits.

ABSTRACT: Transcription factors and their target promoters are central to synthetic biology. By arranging these components into novel gene regulatory circuits, synthetic biologists have been able to create a wide variety of phenotypes, including bistable switches, oscillators, and logic gates. However, transcription factors (TFs) do not instantaneously regulate downstream targets. After the gene encoding a TF is turned on, the gene must first be transcribed, the transcripts must be translated, and sufficient TF must accumulate in order to bind operator sites of the target promoter. The time to complete this process, here called the "signaling time," is a critical aspect in the design of dynamic regulatory networks, yet it remains poorly characterized. In this work, we measured the signaling time of

SUBMITTER: Cheng YY 

PROVIDER: S-EPMC5996764 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature