Formation of Developmentally Toxic Phenanthrene Metabolite Mixtures by Mycobacterium sp. ELW1.

ABSTRACT: Mycobacterium sp. ELW1 co-metabolically degraded up to 1.8 μmol of phenanthrene (PHE) in ∼48 h, and hydroxyphenanthrene (OHPHE) metabolites, including 1-hydroxyphenanthrene (1-OHPHE), 3-hydroxyphenanthrene (3-OHPHE), 4-hydroxyphenanthrene (4-OHPHE), 9-hydroxyphenanthrene (9-OHPHE), 9,10-dihydroxyphenanthrene (1,9-OHPHE), and trans-9,10-dihydroxy-9,10-dihydrophenanthrene (trans-9,10-OHPHE), were identified and quantified over time. The monooxygenase responsible for co-metabolic transformation of PHE was inhibited by 1-octyne. First-order PHE transformation rates, k_PHE, and half-lives, t_1/2, for PHE-exposed cells were 0.16-0.51 h^-1 and 1.4-4.3 h, respectively, and the 1-octyne controls ranged from 0.015-0.10 h^-1 to 7.0-47 h, respectively. While sin

SUBMITTER: Schrlau JE 

PROVIDER: S-EPMC5996983 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature