Notch signaling pathway mediates Doxorubicin-driven apoptosis in cancers.

ABSTRACT:

Background

Doxorubicin is a widely used chemotherapy drug for the treatment of a variety of cancers, however it also has serious side effects such as anaphylaxis and heart damage. Therefore, it's very important to understand the downstream molecular pathways that are essential for Doxorubicin function in cancer treatment.

Methods

HeLa S3 cells were treated with different concentrations of Doxorubicin for 24 hours. Then, the mRNA levels of Notch pathway components in the Doxorubicin treated cells were determined by Real-Time qRT-PCR. Lentiviral transfection was used to up-regulate and down-regulate HES1 expression. Cell proliferation and apoptosis were measured with MTT assay and flow cytometry. Finally, immunofluorescence was used to detect protein subcellular location.

R

SUBMITTER: Huang Z 

PROVIDER: S-EPMC5997178 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature