Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy.

ABSTRACT: The development of technologies to characterize the ocular aqueous outflow system (AOS) is important for the understanding of the pathophysiology of glaucoma. Multiphoton microscopy (MPM) offers the advantage of high-resolution, label-free imaging with intrinsic image contrast because the emitted signals result from the specific biomolecular content of the tissue. Previous attempts to use MPM to image the murine irido-corneal region directly through the sclera have suffered from degradation in image resolution due to scattering of the focused laser light. As a result, transscleral MPM has limited ability to observe fine structures in the AOS. In this work, the porcine irido-corneal angle was successfully imaged through the transparent cornea using a gonioscopic lens to circumvent the highl

SUBMITTER: Masihzadeh O 

PROVIDER: S-EPMC5997970 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature