Circadian clock component PERIOD2 regulates diurnal expression of Na⁺/H⁺ exchanger regulatory factor-1 and its scaffolding function.

ABSTRACT: A number of diverse cell-surface proteins are anchored to the cytoskeleton via scaffold proteins. Na⁺/H⁺ exchanger regulatory factor-1 (NHERF1), encoded by the Slc9a3r1 gene, functions as a scaffold protein, which is implicated in the regulation of membrane expression of various cell-surface proteins. Here, we demonstrate that the circadian clock component PERIOD2 (PER2) modulates transcription of the mouse Slc9a3r1 gene, generating diurnal accumulation of NHERF1 in the mouse liver. Basal expression of Slc9a3r1 was dependent on transcriptional activation by p65/p50. PER2 bound to p65 protein and prevented p65/p50-mediated transactivation of Slc9a3r1. The time-dependent interaction between PER2 and p65 underlay diurnal oscillation in the hepatic expression of Slc9a3r1/

SUBMITTER: Tsurudome Y 

PROVIDER: S-EPMC5998136 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature