Real Time Imaging and Dynamics of Hippocampal Zn²⁺ under Epileptic Condition Using a Ratiometric Fluorescent Probe.

ABSTRACT: Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn²⁺ distribution and its dynamics are essential in view of the fact that Zn²⁺ dyshomeostasis is a risk factor for epileptic seizures, Alzheimer's disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn²⁺ in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn²⁺. The probe is used to detect and quantify the exogenous and endogenous Zn²⁺ in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neur

SUBMITTER: Santhakumar H 

PROVIDER: S-EPMC5998144 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature