Validation of a novel animal model for sciatic nerve repair with an adipose-derived stem cell loaded fibrin conduit.

ABSTRACT: Despite the regenerative capabilities of peripheral nerves, severe injuries or neuronal trauma of critical size impose immense hurdles for proper restoration of neuro-muscular circuitry. Autologous nerve grafts improve re-establishment of connectivity, but also comprise substantial donor site morbidity. We developed a rat model which allows the testing of different cell applications, i.e., mesenchymal stem cells, to improve nerve regeneration in vivo. To mimic inaccurate alignment of autologous nerve grafts with the injured nerve, a 20 mm portion of the sciatic nerve was excised, and sutured back in place in reversed direction. To validate the feasibility of our novel model, a fibrin gel conduit containing autologous undifferentiated adipose-derived stem cells was applied around the coapta

SUBMITTER: Saller MM 

PROVIDER: S-EPMC5998632 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature