Cells with hematopoietic potential reside within mouse proepicardium.

ABSTRACT: During embryonic development, hematopoietic cells are present in areas of blood-vessel differentiation. These hematopoietic cells emerge from a specific subpopulation of endothelial cells called the hemogenic endothelium. We have previously found that mouse proepicardium contained its own population of endothelial cells forming a network of vascular tubules. We hypothesize that this EC population contains cells of hematopoietic potential. Therefore, we investigated an in vitro hematopoietic potential of proepicardial cell populations. The CD31⁺/CD45^-/CD71^- cell population cultured for 10 days in MethocultTM gave numerous colonies of CFU-GEMM, CFU-GM, and CFU-E type. These colonies consisted of various cell types. Flk-1⁺/CD31^-/CD45

SUBMITTER: Jankowska-Steifer E 

PROVIDER: S-EPMC5999137 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature