Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus.

ABSTRACT: Promoter optimization is an economical and effective approach to overexpress heterologous genes and improve the biosynthesis of valuable products. In this study, we swapped the original promoter of the epothilone biosynthetic gene cluster in Myxococcus xanthus with two endogenous strong promoters P _pilA and P _groEL1 , respectively, which, however, decreased the epothilone production ability. The transcriptional abilities by the two promoters were found to be bloomed in the growth stage but markedly decreased after the growth, whereas the original promoter P _epo functioned majorly after the exponential growth stage. Tandem repeat engineering on the original promoter P _epo remarkably increased epothilone production. The tandem promoter exerted simi

SUBMITTER: Yue XJ 

PROVIDER: S-EPMC5999154 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature