[Apoptotic effect of coix polysaccharides on A549 lung cancer cells in vitro].

ABSTRACT:

Background and objective

Coix seeds are commonly used in Traditional Chinese Medicine and ingested through daily diet. The aim of this study is to analyze the apoptotic effect of coix polysaccharides on A549 cells.

Methods

A fraction of polysaccharides was isolated from coix seeds and extracted by ethanol precipitation. The extract was then purified by dialysis and DEAE-52 ion-exchange chromatography. Cell viability was determined by the MTT assay. Cell morphology was observed by scanning electronic microscopy (SEM), and cell cycle was detected by flow cytometry (FCM). The relative quantities of caspase-3 and caspase-9 were determined by RT-PCR.

Results

Coix polysaccharides exerted remarkable inhibitory effects on A549 cell proliferation. Apoptotic bodies were observed

SUBMITTER: Lu X 

PROVIDER: S-EPMC6000036 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature